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Roche rt qpcr reactions
Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
Rt Qpcr Reactions, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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all in one qpcr reaction mix  (Genecopoeia)
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Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
All In One Qpcr Reaction Mix, supplied by Genecopoeia, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt qpcr reaction solutions  (Toyobo)
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Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
Rt Qpcr Reaction Solutions, supplied by Toyobo, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcription polymer ase chain reaction rt pcr kit  (PCR Biosystems Ltd)
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Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
Reverse Transcription Polymer Ase Chain Reaction Rt Pcr Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcription reaction employing hiscript iii rt supermix  (Vazyme Biotech Co)
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Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
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induro rt reaction buffer  (New England Biolabs)
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Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
Induro Rt Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transcription reaction system  (TaKaRa)
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Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
Transcription Reaction System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10x m mulv rt 635 reaction buffer  (New England Biolabs)
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New England Biolabs 10x m mulv rt 635 reaction buffer
Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured <t>by</t> <t>RT-qPCR</t> of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b
10x M Mulv Rt 635 Reaction Buffer, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured by RT-qPCR of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b

Journal: Applied Microbiology and Biotechnology

Article Title: An improved CRISPR-Cas9 protein-based method for knocking out insect Sf9 cell genes

doi: 10.1007/s00253-026-13722-3

Figure Lengend Snippet: Knockout of Sf-Dronc gene: a evaluation by colony-PCR of amplicon size observed in clones D1–D9 and comparison with wild type (Wt) amplicon; b Characterization of cellular response to apoptosis induction by Zeocin™ as assessed by MTT assay and evaluated using GraphPad Prism™; results are shown as average of eight biological replicates ( n = 8), error bars represent standard deviation; c Amplicon coverage of mutants D5 and D8 (genomic sequence) when comparing to amplicon present in wild type cells as seen using IGV™ to visualize Nanopore sequencing data; box highlights the region of interest, featuring the target motif in red; d Relative expression profile measured by RT-qPCR of Sf-Dronc transcripts in mutant populations when compared with wild-type, normalized to Actin-b

Article Snippet: RT-qPCR reactions were performed in a LightCycler 480 Instrument II 384-well block (Roche) and quantification cycle values (Cts), and melting curves were determined using the LightCycler 480 Software version 1.5 (Roche).

Techniques: Knock-Out, Amplification, Clone Assay, Comparison, MTT Assay, Standard Deviation, Sequencing, Nanopore Sequencing, Expressing, Quantitative RT-PCR, Mutagenesis